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Recently, more and more rhodamine derivatives have been used as fluorophores to construct sensors due to their excellent spectroscopic properties. A rhodamine‐based fluorescent and colorimetric Fe 3+  chemosensor 3’,6’‐bis(ethylamino)‐2‐acetoxyl‐2’,7’‐dimethyl‐spiro[1 H ‐isoindole‐1,9’‐[9 H ]xanthen]‐3(2 H )‐one ( RAE ) was designed and synthesized. Upon the addition of Fe 3+ , the dramatic enhancement of both fluorescence and absorbance intensity, as well as the color change of the solution, could be observed. The detection limit of  RAE  for Fe 3+  was around 7.98 ppb. Common coexistent metal ions showed little or no interference in the detection of Fe 3+ . Moreover, the addition of CN −  could quench the fluorescence of the acetonitrile solution of  RAE  and Fe 3+ , indicating the regeneration of the chemosensor  RAE . The robust nature of the sensor was shown by the detection of Fe 3+  even after repeated rounds of quenching. As iron is a ubiquitous metal in cells and plays vital roles in many biological processes, this chemosensor could be developed to have applications in biological studies.

A Highly Selective and Sensitive Turn‐On Fluorescent Chemosensor Based on Rhodamine 6G for Iron(III)