Open AccessRapid Microfluidic Immunoassays of Cancer Biomarker Proteins Using Disposable Inkjet‐Printed Gold Nanoparticle Arrays
Author(s) -
Krause Colleen E.,
Otieno Brunah A.,
Latus Alina,
Faria Ronaldo C.,
Patel Vyomesh,
Gutkind J. Silvio,
Rusling James F.
Publication year - 2013
Publication title -
chemistryopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 29
ISSN - 2191-1363
DOI - 10.1002/open.201300018
Subject(s) - microfluidics , nanotechnology , nanoparticle , colloidal gold , biomarker , cancer biomarkers , materials science , protein detection , cancer , chemistry , medicine , biochemistry
Off‐line amplification: A low‐cost inkjet‐printed gold nanoparticle immunoarray was integrated into a microfluidic device for multiple protein determinations. The use of heavily labeled magnetic beads allows optimization either for ultrasensitive detection of biomarker proteins (low fg mL −1 levels) or for rapid 8 min assays within clinically relevant detection ranges.