Open AccessPhotochemically Active Fluorophore–DNA/RNA Conjugates for Cellular Imaging of Nucleic Acids by Readout in Electron Microscopy
Author(s) -
Holzhauser Carolin,
Kracher Sabrina,
Rubner Moritz M.,
Schmucker Wolfgang,
Wagenknecht HansAchim,
Witzgall Ralph
Publication year - 2013
Publication title -
chemistryopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 29
ISSN - 2191-1363
DOI - 10.1002/open.201300017
Subject(s) - microscopy , fluorophore , fluorescence microscope , sted microscopy , nucleic acid , fluorescence , photoactivated localization microscopy , electron microscope , biomolecule , chemistry , confocal microscopy , biophysics , fluorescence lifetime imaging microscopy , nanotechnology , super resolution microscopy , materials science , biochemistry , biology , optics , stimulated emission , microbiology and biotechnology , physics , laser
A two‐way street: The photochemically active chromophore–nucleic acid conjugates presented herein allow not only imaging by fluorescence microscopy but additionally are able to photoinduce 3,3′‐diaminobenzidine (DAB) polymerization for staining to enable additional readout with electron microscopy.