Open AccessMetal‐Conjugated Affinity Labels: A New Concept to Create Enantioselective Artificial Metalloenzymes
Author(s) -
Reiner Thomas,
Jantke Dominik,
Marziale Alexander N.,
Raba Andreas,
Eppinger Jörg
Publication year - 2013
Publication title -
chemistryopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 29
ISSN - 2191-1363
DOI - 10.1002/open.201200044
Subject(s) - enantioselective synthesis , conjugated system , combinatorial chemistry , chemistry , metal , nanotechnology , organic chemistry , materials science , polymer , catalysis
How to train a protein: Metal‐conjugated affinity labels were used to selectively position catalytically active metal centers in the binding pocket of proteases. The resulting artificial metalloenzymes achieve up to 82 % e.r. in the hydrogenation of ketones. The modular setup enables a rapid generation of artificial metalloenzyme libraries, which can be adapted to a broad range of catalytic conditions.