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Determination of tetrahydrocannabinolic acid—carrier protein conjugate by matrix‐assisted laser desorption/ionization mass spectrometry and antibody formation
Author(s) -
Goto Yuri,
Shima Yumi,
Morimoto Satoshi,
Shoyama Yukihiro,
Murakami Hiroki,
Kusai Akihiko,
Nojima Kazutetsu
Publication year - 1994
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210291115
Subject(s) - chemistry , conjugate , mass spectrometry , matrix assisted laser desorption/ionization , chromatography , hapten , monoclonal antibody , bovine serum albumin , serum albumin , human serum albumin , antigen , desorption , antibody , biochemistry , biology , adsorption , organic chemistry , mathematical analysis , mathematics , immunology , genetics
In order to form the monoclonal antibody against tetrahydrocannabinolic acid (THCA), THCA was coupled with bovine serum albumin (BSA) or human serum albumin (HSA) using β‐alanine as a linker or without β‐alanine to produce individual antigen conjugates. The number of haptens contained in the antigen conjugates was determined by matrix‐assisted laser desorption ionization mass spectrometry using BSA or HSA as an internal standard. The formation of monoclonal antibody is also discussed.