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Electrospray mass spectra of protein cations formed in basic solutions
Author(s) -
Le Blanc J. C. Y.,
Wang Jianyao,
Guevremont Roger,
Siu K. W. M.
Publication year - 1994
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210291103
Subject(s) - chemistry , adduct , electrospray , ion , mass spectrum , mass spectrometry , proton , spectral line , proton affinity , nitrogen , electrospray ionization , yield (engineering) , analytical chemistry (journal) , crystallography , chromatography , organic chemistry , physics , protonation , quantum mechanics , astronomy , materials science , metallurgy
The electrospray mass spectra of gramicidin S cations that originated from 0.2 M solutions of 18 nitrogen‐containing bases were examined. The relative abundances of the [M + 2H] 2+ to the [M + H] + ion were found to correlate not with the solution pH but with the proton affinities of the bases. It is postulated that some of the [M + 2H] 2+ and the [M + H] + ions exist as adducts with the nitrogen bases in solution, these adducts being desorbed into the gas phase during electrospray and dissociated in the lens region via collision‐induced dissociations to yield apparent proton attachment spectra. Some of these adducts were observed under nominally zero collision energy conditions.