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Direct peptide profiling of single neurons by matrix‐assisted laser desorption–ionization mass spectrometry
Author(s) -
van Veelen P. A.,
Jiménez C. R.,
Li K. W.,
Wildering W. C.,
Geraerts W. P. M.,
Tjaden U. R.,
van der Greef J.
Publication year - 1993
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210281229
Subject(s) - mass spectrometry , peptide , lymnaea stagnalis , chemistry , ionization , matrix assisted laser desorption/ionization , desorption , protein mass spectrometry , chromatography , mass spectrum , analytical chemistry (journal) , sample preparation in mass spectrometry , matrix (chemical analysis) , electrospray ionization , biochemistry , ion , biology , ecology , organic chemistry , adsorption , snail
Peptide profiles of single neurons in Lymnaea stagnalis were directly characterized by matrix‐assisted laser desorption–ionization mass spectrometry. The mass analysis was performed after minor pretreatment and without any separation steps. Good‐quality spectra were obtained of several cell types and also other tissues. The results were compared with the results of conventional peptide chemical methods. In addition to many known peptides, several new peptides were identified. The method provides new opportunities for studying peptide compositions at the single‐cell level, which is shown to have many advantages.