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Determination of glycosylation sites in O ‐linked glycopeptides: A sensitive mass spectrometric protocol
Author(s) -
Rademaker Geert Jan,
Haverkamp Johan,
ThomasOates Jane
Publication year - 1993
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210281228
Subject(s) - glycopeptide , glycosylation , chemistry , peptide , mass spectrometry , peptide sequence , glycoprotein , chromatography , amino acid , biochemistry , combinatorial chemistry , gene , antibiotics
Abstract A sensitive mass spectrometric method for the specific identification of O ‐glycosylation sites in glycopeptides is presented. Reductive β‐elimination liberates a peptide containing a specifically modified amino acid at the former site of carbohydrate attachment. Mass spectrometry is used to determine the sequence of the modified peptide, in which the position of the modified amino acid marks the site of O ‐glycosylation. The method also provides information on the carbohydrate chain and is applicable at the low nanomolar level.