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Analysis of conjugated bile acids by on‐line supercritical fluid chromatography/thermospra mass spectrometry
Author(s) -
Scalia Santo,
Games David E.
Publication year - 1992
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210271119
Subject(s) - supercritical fluid chromatography , chromatography , chemistry , mass spectrometry , supercritical fluid , conjugated system , gas chromatography , organic chemistry , polymer
A rapid method was developed for the analysis or polar conjugated bile acids by combined packed column super‐critical fluid chromatography/mass spectrometry. Direct coupling of the column to the mass spectrometer was effected with a modified thermospray interface operated in the filament‐on mode. Maximum sensitivity for the bile acid conjugates was achieved by recording the negative ions generated in the ionization process. The effects of vaporizer and source temperatures, repeller voltage and discharge conditions on the bile acid response were investigated. The mass spectra obtained for the common glycine and taurine conjugates yielded only the [M – H] − pseudo‐molecular ions. In contrast with the taurine derivatives, the glycine forms produced a weak ion current and exhibited broad hands. The applicability of the technique is illustrated with a sample of human bile.