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Single‐photon and multi‐photon ionization of infrared laser‐desorbed biomolecules
Author(s) -
Köster Claus,
Grotemeyer Jürgen
Publication year - 1992
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210270418
Subject(s) - photoionization , ionization , chemistry , reflectron , atmospheric pressure laser ionization , mass spectrometry , matrix assisted laser desorption electrospray ionization , infrared , ion , time of flight mass spectrometry , optics , physics , electron ionization , chromatography , organic chemistry
Results from investigations of the 10.5 eV photoionization of infrared laser‐desorbed, jet‐entrained peptides are presented. The instrumental set‐up for vacuum ultraviolet generation and the coupling to a reflectron‐time‐of‐flight mass spectrometer is described. Single‐photon ionization makes possible the investigation of molecules without any chromophoric group. Examples of the application of this ionization method to di‐ and tripeptides containing glycine, alanine, leucine and proline are shown. A comparison between single‐ and multi‐photon ionization of tryptophan and tryptophar‐containing peptides up to four amino acids is made and discussed in terms of soft ionization and generation of fragment ions.