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Investigation of lacidipine, a new 1,4‐dihydropyridine antihypertensive agent and three chemically related compounds by means of chemical ionization, mass spectrometry and collision‐induced dissociation
Author(s) -
Harridan M.,
Scandola M.,
Franchi D.,
Gaviraghi G.,
Tarzia G.
Publication year - 1992
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210270315
Subject(s) - chemistry , protonation , collision induced dissociation , dissociation (chemistry) , mass spectrum , mass spectrometry , reagent , chemical ionization , ion , lacidipine , ionization , analytical chemistry (journal) , molecule , tandem mass spectrometry , chromatography , organic chemistry , biochemistry , receptor , antagonist
Chemical ionization of two 1,4‐dihydropyridines, lacidipine and its Z ‐isomer, and their corresponding pyridines in three different reagent gases and the collision‐induced dissociation (CID) of their respective mass‐selected protonated molecular ions in the collision energy range 10–200 eV were performed on a multiple quadrupole instrument. The weakness of the Breasted acid NH 4 + as a protonating agent is clearly manifested in one of the ammonia positive‐ion chemical ionization (CI + ) mass spectra which displays the addition ion, [M + NH 4 ] + , as the favoured reaction channel. The stereochemistry of the precursor molecules, the exothermicity of the protonation process and the threshold of certain dissociation channels as a function of the collision energy are among the arguments invoked to explain some of the observed differences between the CI + mass spectra and the CID data of the different isomers investigated. In an attempt to present a more comprehensive study, some high‐performance liquid chromatographic retention times and resolutions are also given.