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Mechanism of formation of alkali metal cation adducts in plasma desorption mass spectrometry of biomolecules
Author(s) -
Demirev P.,
Fenyö D.,
Håkansson P.,
Sundqvist B. U. R.
Publication year - 1991
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210260518
Subject(s) - chemistry , mass spectrometry , alkali metal , desorption , adduct , protonation , ion , biomolecule , analytical chemistry (journal) , ionic bonding , molecule , chromatography , organic chemistry , adsorption , biochemistry
Abstract Experimental data on correlations between the directions of the incident primary ion and the ejected protonated [M + H] + and alkali metal adduct [M + Cs] + molecules for three peptide samples with an incident beam of 72.3 MeV 127 I 14+ ions are reported. Measurements were carried out in a linear time‐of‐flight mass spectrometer by monitoring the secondary ion yield as a function of electrostatic deflection in a direction perpendicular to the spectrometer axis. No difference was observed in the direction in which [M + H] + and [M + Cs] + ionic species are preferentially desorbed. The results obtained suggest that alkali metal cation attachment to biomolecules in plasma desorption mass spectrometry is realized in a close spatial location and time interval with protonation. Formation of ion‐molecule complexes occurs at an early stage of the desorption event and precedes their ejection into the gas phase.