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Tuning and calibration in thermospray liquid chromatography/mass spectrometry using perfluorinated alkyl acids and their ammonium salts
Author(s) -
Stout Steven J.,
daCunha Adrian R.
Publication year - 1990
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210250402
Subject(s) - thermospray , chemistry , mass spectrometry , alkyl , trifluoroacetic acid , ion , calibration , chromatography , ammonium , ammonium acetate , analytical chemistry (journal) , inorganic chemistry , tandem mass spectrometry , organic chemistry , high performance liquid chromatography , selected reaction monitoring , statistics , mathematics
Of the several approaches used to interface liquid chromatography with mass spectrometry, the thermospray interface has evolved as the most popular and applicable for the analysis of polar and labile organic compounds. Since a separate ion source must be used for thermospray, separate tuning and calibration are required. Unfortunately, current approaches to tuning and calibration suffer from serious shortcomings, most notably rapid contamination of the ion source. Recent reports have shown that this shortcoming can be overcome by using acetic acid‐ammonia cluster ions or trifluoroacetic acid–ammonia cluster ions for tuning and calibration. In the latter approach, the tuning solution could also be used in the negative ion mode of operation but suffers from concentration of most of the ion current in one ion. The use of perfluorinated alkyl acids and their ammonium salts to generate intense high‐mass negative ions for tuning and calibration to m/z 2000 is reported. Additionally, the ammonium salts of the longer chain perfluorinated acids offer an enhanced high‐mass response in the positive ion mode of operation.