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Influence of reversed‐ and normal‐phase liquid chromatographic eluents in the fragmentation of selected chlorinated herbicides in thermospray liquid chromatography‐mass spectrometry
Author(s) -
Barceló Damià
Publication year - 1989
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210241009
Subject(s) - thermospray , chemistry , ammonium acetate , chromatography , acetonitrile , cyclohexane , mass spectrometry , ammonium , ethyl acetate , high performance liquid chromatography , selected reaction monitoring , organic chemistry , tandem mass spectrometry
The effect of two completely different mobile phase compositions, reversed‐phase acetonitrile‐water + ammonium acetate and normal‐phase cyclohexane, were compared in filament‐on thermospray liquid chromatography‐mass spectrometry (LC‐MS) for the determination of selected chlorinated herbicides such as chloroatrazines and chlorinated phenoxyacetic acids. By using acetonitrile‐water + 0.05 M ammonium acetate mixtures in positive ion mode thermospray LC‐MS, the chloroatrazine herbicides showed the acetonitrile adduct ion [M + (CH 3 CN)H] + as the base peak, whereas the chlorinated phenoxyacetic acids showed no signal. In contrast, when cyclohexane, which is reported for the first time as an eluent in the thermospray technique, was used as the mobile phase the chlorinated phenoxyacetic acid herbicides exhibited [M – H] + , [M – Cl] + and M + ˙ as the main ions. Negative ion mode thermospray LC‐MS showed [M – H] − as the base peak for the chloroatrazines in the different mobile phases, whereas the chlorinated phenoxyacetic acids exhibited [M + H] − , [M + Cl] − or [M – HCl] − as the base peaks in cyclohexane and [M + acetate] − in acetonitrile‐water‐ammonium acetate.