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Application of on‐line HPLC‐FAB mass spectrometry to the analysis of enzymatic digests of ribonuclease B
Author(s) -
Mock K.,
Firth J.,
Cottrell J. S.
Publication year - 1989
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210240813
Subject(s) - chromatography , mass spectrometry , ribonuclease , chemistry , trypsin , high performance liquid chromatography , slurry , enzyme , biochemistry , materials science , rna , gene , composite material
A tryptic digest of bovine ribonuclease B was analysed by on‐line HPLC‐FAB mass spectrometry to verify the primary sequence. Peptides obtained by digestion with trypsin before and after treatment with N ‐glycanase were separated on a slurry‐packed fused silica LC column. The entire eluent was introduced into the mass spectrometer source via a continuous flow interface. A single injection of 100 pinoles of material provided data covering over 70% of the sequence.