Application of on‐line HPLC‐FAB mass spectrometry to the analysis of enzymatic digests of ribonuclease B | Zendy

Mock K. | Zendy; Firth J. | Zendy; Cottrell J. S. | Zendy

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Application of on‐line HPLC‐FAB mass spectrometry to the analysis of enzymatic digests of ribonuclease B

Author(s) -

Mock K.,

Firth J.,

Cottrell J. S.

Publication year - 1989

Publication title -

organic mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 0030-493X

DOI - 10.1002/oms.1210240813

Subject(s) - chromatography , mass spectrometry , ribonuclease , chemistry , trypsin , high performance liquid chromatography , slurry , enzyme , biochemistry , materials science , rna , gene , composite material

A tryptic digest of bovine ribonuclease B was analysed by on‐line HPLC‐FAB mass spectrometry to verify the primary sequence. Peptides obtained by digestion with trypsin before and after treatment with N ‐glycanase were separated on a slurry‐packed fused silica LC column. The entire eluent was introduced into the mass spectrometer source via a continuous flow interface. A single injection of 100 pinoles of material provided data covering over 70% of the sequence.

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