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Mass spectra of doubly charged ions
Author(s) -
Barber Michael,
Bell David J.,
Morris Michael,
Tetler Lee W.,
Woods Michael D.,
Monaghan John J.,
Morden William E.
Publication year - 1989
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210240711
Subject(s) - ion , chemistry , mass spectrometry , mass spectrum , molecule , tandem mass spectrometry , protonation , spectral line , charged particle , analytical chemistry (journal) , atomic physics , physics , chromatography , organic chemistry , astronomy
The mass spectra of biological molecules, whose molecular mass exceeds 10 kDa, invariably contain multiply charged ions. For example, a survey scan of a small protein will produce singly, doubly and triply protonated molecules, the intensity of the doubly charged species often being greater than that of the singly charged entity. Although the spectra resulting from doubly charged peptides have not previously been studied, collisional activation of such doubly charged species may result in significant additional information pertaining to molecular structure. The techniques employed to study ions originating from multiply charged species were linked scanning of constant B/E and tandem mass spectrometry, namely low collision energy spectra acquired on a BEQQ hybrid instrument. The methodology was applied to model compounds whose tandem mass spectrometry characteristics are well known, e.g. gramicidin S and angiotensin I. The results for the product ions of the [M + 2H] 2+ species of the models were obtained which highlight the methodology required for high‐mass materials.