Premium
A general soft ionization method for mass spectrometry: Resonance‐enhanced multi‐photon ionization of biomolecules
Author(s) -
Grotemeyer Jürgen,
Boesl Ulrich,
Walter Klaus,
Schlag Edward W.
Publication year - 1986
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210211008
Subject(s) - biomolecule , mass spectrometry , ionization , reflectron , resonance (particle physics) , chemistry , atmospheric pressure laser ionization , desorption , photoionization , mass spectrometry imaging , time of flight mass spectrometry , analytical chemistry (journal) , atomic physics , physics , chromatography , ion , adsorption , biochemistry , organic chemistry
We show here that ‘double‐barrelled’ laser desorption/resonance‐enhanced multi‐photon ionization mass spectrometry has many advantages over traditional single‐step desorption/ionization techniques, particularly if combined with Reflectron‐Time‐of‐Flight mass analysers. We demonstrate the effectiveness of this technique for large labile biomolecules, namely native chlorophylls, porphyrins and a peptide, as examples of structural and mass analysis.