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Chemical ionization mass spectrometry of complex molecules—VI: Peptides
Author(s) -
Kiryushkin A. A.,
Fales H. M.,
Axenrod T.,
Gilbert E. J.,
Milne G. W. A.
Publication year - 1971
Publication title -
organic mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0030-493X
DOI - 10.1002/oms.1210050104
Subject(s) - electron ionization , chemistry , mass spectrum , mass spectrometry , ion , chemical ionization , molecule , ionization , peptide , sequence (biology) , peptide sequence , carbonium ion , spectral line , amino acid , peptide bond , analytical chemistry (journal) , computational chemistry , organic chemistry , chromatography , biochemistry , physics , astronomy , gene
The chemical ionization mass spectra of a series of simple peptides containing six or fewer amino acids have been studied. Using methane as the reactant gas we found cleavage of the peptide bond occurs in two ways, yielding either the acyl carbonium ion or the complementary ammonium ion. The observation of both types of fragments permits the determination of the amino acid sequence of the peptide. The ammonium ions provide an additional sequence determining route compared to that available from electron‐impact spectra. ‘Sequence‐determing ions,’ especially the quasimolecular ion at m/e [M+1] are usually more intense than in the electron‐impact mass spectra.