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Adipocyte Proteins and Storage of Endogenous Fatty Acids in Visceral and Subcutaneous Adipose Tissue in Severe Obesity
Author(s) -
Lytle Kelli A.,
Bush Nikki C.,
Triay Jessica M.,
Kellogg Todd A.,
Kendrick Michael L.,
Swain James M.,
Gathaiya Nicola W.,
Hames Kazanna C.,
Jensen Michael D.
Publication year - 2021
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.23149
Subject(s) - very low density lipoprotein , medicine , endocrinology , adipose tissue , chemistry , lipoprotein lipase , adipose triglyceride lipase , triglyceride , fatty acid , lipolysis , lipoprotein , biochemistry , biology , cholesterol
Objective This study tested whether substrate concentrations or fatty acid storage proteins predict storage of endogenous lipids in visceral adipose tissue (VAT) and upper body subcutaneous adipose tissue (UBSQ) fat. Methods The day prior to surgery, 25 patients undergoing bariatric procedures received an infusion of autologous [1‐ 14 C]triolein‐labeled very low‐density lipoprotein (VLDL) particles, and during surgery, they received a continuous [U‐ 13 C]palmitate infusion/bolus [9,10‐ 3 H]palmitate tracer. VAT and UBSQ fat were collected to measure VLDL‐triglyceride (TG) storage, direct free fatty acid (FFA) storage rates, CD36 content, lipoprotein lipase (LPL), acyl‐CoA synthetase, diacylglycerol acetyl‐transferase, and glycerol‐3‐phosphate acyltransferase activities. Results Storage of VLDL‐TG and FFA‐palmitate in UBSQ and VAT was not different. Plasma palmitate concentrations correlated with palmitate storage rates in UBSQ and VAT ( r = 0.46, P = 0.02 and r = 0.46, P = 0.02, respectively). In VAT, VLDL‐TG storage was correlated with VLDL concentrations ( r = 0.53, P < 0.009) and LPL ( r = 0.42, P < 0.05). In UBSQ, VLDL‐TG storage was correlated with LPL ( r = 0.42, P < 0.05). CD36, acyl‐CoA synthetase, glycerol‐3‐phosphate acyltransferase, and diacylglycerol acetyl‐transferase were not correlated with VLDL‐TG or palmitate storage. Conclusions Adipose storage of VLDL‐TG is predicted by VLDL‐TG concentrations and LPL; FFA concentrations predict direct adipose tissue FFA storage rates.