Premium
Different Expression of Duodenal Genes Related to Insulin Resistance Between Nonobese Women and Those with Severe Obesity
Author(s) -
HoPlagaro Ailec,
SantiagoFernandez Concepción,
RodríguezDíaz Cristina,
LopezGómez Carlos,
GarciaSerrano Sara,
RodríguezPacheco Francisca,
Valdes Sergio,
RodríguezCañete Alberto,
AlcaínMartínez Guillermo,
RuizSantatalia,
VázquezPedreño Luis,
GarcíaFuentes Eduardo
Publication year - 2020
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.22902
Subject(s) - insulin resistance , endocrinology , medicine , downregulation and upregulation , insulin , lipid metabolism , inflammation , biology , immune system , immunology , gene , biochemistry
Objective The study aim was to identify changes in duodenal gene expression associated with the development of insulin resistance according to the BMI of women. Methods Duodenal samples were assessed by microarray in four groups of women, nonobese women and women with severe obesity, with both low and high insulin resistance. Results There was a group of shared downregulated differentially expressed genes (DEGs) related to tissue homeostasis and antimicrobial humoral response in women with higher insulin resistance both with severe obesity and without obesity. In the exclusive DEGs found in severe obesity, downregulated DEGs related to the regulation of the defense response to bacterium and cell adhesion, involving pathways related to the immune system, inflammation, and xenobiotic metabolism, were observed. In the exclusive DEGs from nonobese women with higher insulin resistance, upregulated DEGs mainly related to the regulation of lipoprotein lipase activity, very low‐density lipoprotein particle remodeling, lipid metabolic process, antigen processing, and the presentation of peptide antigen were found. Conclusions Independent of BMI, higher insulin resistance was associated with a downregulation of duodenal DEGs mainly related to the immune system, inflammation, and xenobiotic metabolism. Also, intestinal lipoprotein metabolism may have a certain relevance in the regulation of insulin resistance in nonobese women.