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Pentraxin 3 Regulates miR‐21 Expression and Secretion in Brown Adipocytes During Lipopolysaccharide‐Induced Inflammation
Author(s) -
Lin Teyueh,
Guo Hong,
Chen Xiaoli
Publication year - 2020
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.22701
Subject(s) - ptx3 , downregulation and upregulation , inflammation , lipopolysaccharide , secretion , stromal cell , endocrinology , stimulation , adipose tissue , medicine , tumor necrosis factor alpha , biology , microbiology and biotechnology , chemistry , biochemistry , gene
Objective This study investigates the role of pentraxin 3 (PTX3) in the regulation of inflammatory homeostasis involving anti‐inflammatory miR‐21 during lipopolysaccharide (LPS) stimulation in adipocytes. Methods Using PTX3 knockout (PTX3 KO) mouse and primary stromal vascular cell models, this study determined the effect of PTX3 deficiency on the expression and secretion of miR‐21 in brown adipose tissue (BAT) and brown adipocytes as well as the rescue effect of recombinant PTX3 on miR‐21 during LPS‐induced inflammation. Results Among three fat depots, BAT was the major tissue and fully differentiated brown adipocytes were the major cells that expressed miRNA‐processing enzymes and produced miRNA. Moreover, brown adipocytes, but not stromal vascular cells, were the LPS‐responsive cells in miR‐21 production. PTX3 deficiency attenuated LPS‐stimulated upregulation of miR‐21 in sera and BAT. In wild‐type brown adipocytes, LPS stimulation significantly upregulated cellular miR‐21. Interestingly, this stimulatory effect of LPS was attenuated, and cellular and secreted miR‐21 levels were reduced in PTX3 KO cells upon LPS stimulation. Treatment of recombinant PTX3 reversed the cellular and secreted levels of miR‐21 and attenuated an LPS‐stimulated increase in the expression of Tnf‐α and Mcp1 genes in PTX3 KO adipocytes. Conclusions PTX3 plays an anti‐inflammatory role, in part through regulating miR‐21 expression and secretion.