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Genetically Engineered Escherichia coli Nissle 1917 Secreting GLP‐1 Analog Exhibits Potential Antiobesity Effect in High‐Fat Diet‐Induced Obesity Mice
Author(s) -
Ma Jie,
Li Cuiying,
Wang Junrui,
Gu Jianwen
Publication year - 2020
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.22700
Subject(s) - endocrinology , medicine , hypothalamus , obesity , neuropeptide , neuropeptide y receptor , diet induced obese , body weight , biology , insulin resistance , receptor
Objective This study aimed to investigate the potential antiobesity effect of genetically modified Escherichia coli Nissle 1917 (EcN‐GM) in mice fed a high‐fat diet (HFD). Methods The mice were randomly divided into six groups: a normal diet group (ND), a HFD group, a HFD + EcN group, and three HFD + EcN‐GM groups. The effects of EcN‐GM on body weight, food intake, fat pad and organ weight, and an oral glucose tolerance test were measured, in addition to hepatic biochemistry and histological analysis. The mRNA expression of neuropeptides related to food intake regulation in the hypothalamus was also detected. Results The results showed that EcN‐GM decreased body weight, body weight gain, food intake, fat pad weight, and hepatic weight of HFD mice. There were beneficial effects of EcN‐GM on blood glucose, hepatic biochemistry, and hepatic histological alterations. A dramatic switch of food intake‐regulating gene expression in the hypothalamus was also observed in mice. Conclusions This work has revealed that a modified live bacterial therapeutic, EcN‐GM, has potential beneficial effects on obesity. This effect may be related to the regulating of the neuropeptide expression of energy intake and expenditure in the hypothalamus. This study demonstrates a successful example of engineered EcN‐GM as a novel approach for weight management.