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Fibroblast Growth Factor 21 Exerts its Anti‐inflammatory Effects on Multiple Cell Types of Adipose Tissue in Obesity
Author(s) -
Wang Nan,
Zhao Tingting,
Li Siming,
Sun Xu,
Li Zicheng,
Li Yanhua,
Li Deshan,
Wang Wenfei
Publication year - 2019
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.22376
Subject(s) - adipose tissue , fgf21 , obesity , fibroblast growth factor , fibroblast , medicine , growth factor , cell type , endocrinology , cell , biology , receptor , in vitro , biochemistry
Objective Obesity‐related, chronic, low‐grade inflammation has been identified as a key factor in the development of many metabolic diseases, such as type 2 diabetes and cardiovascular diseases. Adipocytes, preadipocytes, and macrophages have been implicated in initiating inflammation in adipose tissue. This study aims to investigate the effects of fibroblast growth factor‐21 (FGF‐21) on obesity‐related inflammation and its mechanisms in vivo and in vitro .Methods Monosodium glutamate (MSG) was used to induce obesity in mice and subsequently treated the mice with or without FGF‐21. Primary adipocytes and stromal vascular fraction cells were isolated from MSG‐obesity mice for additional experiments.Results Results obtained by ELISA and real‐time polymerase chain reaction showed that FGF‐21 efficiently ameliorated obesity‐related inflammation in MSG‐obesity mice. This study demonstrated that preadipocytes and adipocytes responded to anti‐inflammatory effects of FGF‐21. In vitro , 3 T3‐L1 preadipocytes lacking β‐klotho did not respond to FGF‐21 under glucose uptake. Interestingly, the treatment of 3 T3‐L1 preadipocytes with FGF‐21 significantly attenuated lipopolysaccharide‐induced inflammatory response.Conclusions Our study showed that FGF‐21–induced glucose uptake and FGF‐21–related anti‐inflammatory effects are mediated by different signaling pathways. Moreover, FGF‐21 showed anti‐inflammatory effects on preadipocytes; these effects are mediated by the fibroblast growth factor receptor substrate 2/ERK1/2 signaling pathway.