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CD Obesity‐Prone Rats, but not Obesity‐Resistant Rats, Robustly Ferment Resistant Starch Without Increased Weight or Fat Accretion
Author(s) -
Obanda Diana,
Page Ryan,
Guice Justin,
Raggio Anne M.,
Husseneder Claudia,
Marx Brian,
Stout Rhett W.,
Welsh David A.,
Taylor Christopher M.,
Luo Meng,
Blanchard Eugene E.,
Bendiks Zach,
Coulon Diana,
Keenan Michael J.
Publication year - 2018
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.22120
Subject(s) - obesity , fermentation , composition (language) , gut flora , weight loss , starch , resistant starch , food science , biology , endocrinology , medicine , chemistry , biochemistry , philosophy , linguistics
Objective This study used CD obesity‐prone (OP) and obesity‐resistant (OR) rats to examine how weight gain and fat accretion relate to fermentation levels and microbiota composition after feeding resistant starch (RS). Methods After feeding OP rats and OR rats a high‐fat (HF) diet for 4 weeks, rats were stratified into three groups: they were fed either an HF diet (group 1: HF‐HF) or were switched to a low‐fat (LF) diet (group 2: HF‐LF) or an LF diet supplemented with 20% RS by weight for 4 weeks (group 3: HF‐LFRS). Energy intake, body weight, fermentation variables, and microbiota composition were determined. Results In OP rats, RS elicited robust fermentation (increased cecal contents, short‐chain fatty acids, and serum glucagon‐like peptide 1). Total bacteria, species of the Bacteroidales family S24‐7 , and the archaean Methanobrevibacter smithii increased. The robust fermentation did not elicit higher weight or fat accretion when compared with that of control rats fed the same isocaloric diets (HF‐LF ± RS). In OR rats, body weight and fat accretion were also not different between HF‐LF ± RS diets, but RS elicited minimal changes in fermentation and microbiota composition. Conclusions Robust fermentation did not contribute to greater weight. Fermentation levels and changes in microbiota composition in response to dietary RS differed by obesity phenotype.