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Upregulation of Foreign Body Response in Obese Mice
Author(s) -
Orellano Laura Alejandra Ariza,
de Almeida Simone Aparecida,
Pereira Luciana Xavier,
Couto Letícia Chinait,
de Lazari Marcela Guimarães Takahashi,
Viana Celso Tarso Rodrigues,
Andrade Silvia Passos,
Campos Paula Peixoto
Publication year - 2018
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.22102
Subject(s) - capsule , downregulation and upregulation , implant , foreign body , medicine , transforming growth factor , obesity , fibroblast , tissue engineering , endocrinology , pathology , surgery , chemistry , biology , biomedical engineering , cell culture , biochemistry , botany , genetics , gene
Objective Obesity is a highly prevalent multifactorial metabolic condition in which the need for functional bioengineered substitutes (e.g., scaffolds for tissue engineering) is likely to occur. However, the adverse foreign body response (FBR) that invariably takes place adjacent to implant devices impairing their function is poorly characterized in this condition. This study investigated the influence of obesity on the host response to a synthetic matrix implanted subcutaneously in high‐fat‐fed obese mice. Methods Histological analysis of 14‐day‐old implants was performed to identify collagen deposition, capsule thickness, fibroblast‐like cells, foreign body giant cells, and mast cells. In addition, transforming growth factor β1 (TGF‐β1) levels in the implants and serum were determined. Results All fibrogenic markers (and TGF‐β1 levels) increased in the implants of obese mice compared with their nonobese counterparts. Particularly relevant was the fibrous capsule thickness in implants of obese mice (234.2 ± 22.1 µm vs. 109.2 ± 13.4 µm in implants of nonobese animals). Conclusions The study results showing that obesity upregulates the main features of the FBR induced by subcutaneous implants in mice may be relevant in understanding biomaterial integration and performance in this condition. This is crucial to the development of strategies to maintain the integrity and function of implantable devices.