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Morphological and Functional Changes in the Peritumoral Adipose Tissue of Colorectal Cancer Patients
Author(s) -
Zoico Elena,
Rizzatti Vanni,
Darra Elena,
Budui Simona Luciana,
Franceschetti Guido,
Vinante Fabrizio,
Pedrazzani Corrado,
Guglielmi Alfredo,
De Manzoni Giovanni,
Mazzali Gloria,
Rossi Andrea Petronio,
Fantin Francesco,
Zamboni Mauro
Publication year - 2017
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.22008
Subject(s) - adipose tissue , adiponectin , lipolysis , cd68 , cd163 , colorectal cancer , medicine , adipose tissue macrophages , immunohistochemistry , adipocyte , macrophage , pathology , adipokine , cancer , inflammation , endocrinology , cancer research , in vitro , white adipose tissue , biology , obesity , leptin , insulin resistance , biochemistry
Objective The role of peritumoral adipose tissue (AT) has not been extensively studied in colorectal cancer (CRC). Methods This study was conducted in 20 male subjects undergoing elective surgery for CRC. The differences between the peritumoral visceral adipose tissue (P‐VAT), visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) of the patients were described via immunohistochemistry and molecular biology analyses. The interactions between adipocytes and a colon cancer cell line were also investigated by using an in vitro coculture system. Results The analyses revealed that adipocytes near the tumor were significantly smaller than the adipocytes from other sites. The P‐VAT was preferentially infiltrated by a CD68+/CD163+/IDO‐ macrophage subset with a prevalent reparative inflammatory response, while the macrophages identified in VAT and SAT mainly presented inflammatory features. Furthermore, the P‐VAT presented a higher expression of adiponectin compared with other sites. Morphological analysis in vitro showed that after a few days of coculture, 3T3‐L1 adipocytes were reduced in number and size with an increase in lipolysis rate and dedifferentiation phenomena. Conclusions This study reveals important morphological and functional changes in the AT surrounding the tumor as an increase in lipolysis and in adiponectin‐producing adipocytes, preferentially infiltrated by a macrophage subset, with prevalent reparative inflammatory response.