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A combination of resveratrol and quercetin induces browning in white adipose tissue of rats fed an obesogenic diet
Author(s) -
Arias Noemí,
Picó Catalina,
Teresa Macarulla M.,
Oliver Paula,
Miranda Jonatan,
Palou Andreu,
Portillo María P.
Publication year - 2017
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.21706
Subject(s) - white adipose tissue , brown adipose tissue , browning , endocrinology , resveratrol , adipose tissue , medicine , quercetin , thermogenin , western blot , immunostaining , fgf21 , prdm16 , diet induced obese , chemistry , obesity , biology , immunohistochemistry , biochemistry , insulin resistance , antioxidant , receptor , gene , fibroblast growth factor
Objective To analyze whether a combination of quercetin (Q) and resveratrol (RSV) would induce a white adipose tissue (WAT) browning effect. Methods Thirty‐six rats were fed an obesogenic diet and divided into four groups: control, treated with RSV (15 mg/kg body weight/day; RSV group), treated with Q (30 mg/kg body weight/day; Q group), or treated with both polyphenols (RSV + Q group). Results After 6 weeks, body and WAT weights were significantly reduced in the RSV + Q group. In perirenal WAT of the control, RSV, and Q groups, white unilocular adipocytes appeared in the majority of cells, while in the RSV + Q group numerous multilocular adipocytes with positive immunostaining for UCP1 were observed. The presence of UCP1 was confirmed by Western blot. This group also revealed increased mRNA levels of Cidea , Hocx9, Bmp4, Slc27a1 , Pat2 , Atgl , and Atp5d . Interscapular brown adipose tissue weight showed no differences between groups, but the Cidea mRNA level was increased in the RSV group, the Cox‐2 mRNA level in the RSV + Q group, and UCP1 protein expression in the RSV and the RSV + Q groups. Conclusions This study demonstrated that the RSV + Q combination produces a brown‐like remodeling effect in perirenal WAT, as well as increased UCP1 protein expression in interscapular brown adipose tissue.