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Xanthoangelol and 4‐hydroxyderrcin suppress obesity‐induced inflammatory responses
Author(s) -
Li Yongjia,
Goto Tsuyoshi,
Ikutani Ryuma,
Lin Shan,
Takahashi Nobuyuki,
Takahashi Haruya,
Jheng HueiFen,
Yu Rina,
Taniguchi Masahiko,
Baba Kimiye,
Murakami Shigeru,
Kawada Teruo
Publication year - 2016
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.21611
Subject(s) - inflammation , adipocyte , adipose tissue , insulin resistance , endocrinology , medicine , pathogenesis , activator (genetics) , white adipose tissue , in vivo , chemistry , adipose tissue macrophages , phosphorylation , transcription factor , obesity , biology , biochemistry , gene , receptor , microbiology and biotechnology
Objective Obesity‐induced inflammation plays a pivotal role in the pathogenesis of insulin resistance and type 2 diabetes. Xanthoangelol (XA) and 4‐hydroxyderrcin (4‐HD), phytochemicals extracted from Angelica keiskei , have been reported to possess various biological properties. Whether XA and 4‐HD alleviate obesity‐induced inflammation and inflammation‐induced adipocyte dysfunction was investigated. Methods For the in vitro study, a co‐culture system composed of macrophages and adipocytes and macrophages stimulated with conditioned medium derived from fully differentiated adipocytes was conducted. For the in vivo study, mice were fed a high‐fat diet supplemented with XA for 14 weeks. Results XA and 4‐HD suppressed inflammatory factors in co‐culture system. Moreover, treatment of RAW macrophages with XA and 4‐HD moderated the suppression of uncoupling protein 1 promoter activity and gene expression in C3H10T1/2 adipocytes, which was induced by conditioned medium derived from LPS‐stimulated RAW macrophages. Also, XA and 4‐HD inhibited c‐Jun N‐terminal kinase phosphorylation, nuclear factor‐κB, and activator protein 1, the last two being transcription activators in activated macrophages. Furthermore, in mice fed the high‐fat diet, XA reduced inflammatory factors within the white adipose tissue. Conclusions These results suggest that XA and 4‐HD might be promising phytochemicals to suppress obesity‐induced inflammation and inflammation‐induced adipocyte dysfunction.