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Adipose tissue fibrosis, hypertrophy, and hyperplasia: Correlations with diabetes in human obesity
Author(s) -
Muir Lindsey A.,
Neeley Christopher K.,
Meyer Kevin A.,
Baker Nicki A.,
Brosius Alice M.,
Washabaugh Alexandra R.,
Varban Oliver A.,
Finks Jonathan F.,
Zamarron Brian F.,
Flesher Carmen G.,
Chang Joshua S.,
DelProposto Jennifer B.,
Geletka Lynn,
MartinezSantibanez Gabriel,
Kaciroti Niko,
Lumeng Carey N.,
O'Rourke Robert W.
Publication year - 2016
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.21377
Subject(s) - adipose tissue , adipocyte , fibrosis , medicine , hyperplasia , endocrinology , context (archaeology) , diabetes mellitus , muscle hypertrophy , obesity , biology , paleontology
Objective The relationship between adipose tissue fibrosis, adipocyte hypertrophy, and preadipocyte hyperplasia in the context of obesity and the correlation of these tissue‐based phenomena with systemic metabolic disease are poorly defined. The goal of this study was to clarify the relationship between adipose tissue fibrosis, adipocyte hypertrophy, and preadipocyte hyperplasia in human obesity and determine the correlation of these adipose‐tissue based phenomena with diabetes. Methods Visceral and subcutaneous adipose tissues from humans with obesity collected during bariatric surgery were studied with QRTPCR, immunohistochemistry, and flow cytometry for expression of collagens and fibrosis‐related proteins, adipocyte size, and preadipocyte frequency. Results were correlated with clinical characteristics including diabetes status. Results Fibrosis was decreased, hypertrophy was increased, and preadipocyte frequency and fibrotic gene expression were decreased in adipose tissues from diabetic subjects compared to non‐diabetic subjects. These differences were greater in visceral compared to subcutaneous adipose tissue. Conclusions These data are consistent with the hypothesis that adipose tissue fibrosis in the context of human obesity limits adipocyte hypertrophy and is associated with a reciprocal increase in adipocyte hyperplasia, with beneficial effects on systemic metabolism. These findings suggest adipose tissue fibrosis as a potential target for manipulation of adipocyte metabolism.