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Modulation of IL ‐27 in adipocytes during inflammatory stress
Author(s) -
Nam Heesun,
Ferguson Bradley S.,
Stephens Jacqueline M.,
Morrison Ron F.
Publication year - 2016
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.21351
Subject(s) - inflammation , autocrine signalling , paracrine signalling , cytokine , adipose tissue , stimulation , secretion , endocrinology , proinflammatory cytokine , interleukin 6 , interleukin , medicine , immunology , biology , microbiology and biotechnology , chemistry , receptor
Objective While it is well established that adipose tissue‐derived inflammation plays an important role in the pathogenic mechanisms linking obesity with metabolic dysfunction, the inflammatory mediators involved have not been fully elucidated. Here, we explored IL‐12 family cytokines with a focus on IL‐27 during obesity‐induced inflammation in mice and cultured adipocytes (ADs) following exposure to inflammatory stimuli. Methods Relative mRNA abundance of IL‐12 cytokines was assessed by reverse transcription polymerase chain reaction (RT‐PCR) in genetically obese B6‐ob/ob mice as well as C57BL/6J mice fed a high‐fat diet and in ADs following exposure to inflammatory stimuli. Protein secretion of cytokines into culture media was assessed by ELISA, and the biological outcome of IL‐27 stimulation was assessed by RT‐PCR and immunoblotting. Results Heterodimeric subunits constituting IL‐27 were significantly induced in obese mice. While all IL‐12 genes were markedly induced by inflammatory stress in cultured ADs, IL‐27 protein was the only cytokine secreted into culture media in response to inflammatory stress. Cultured ADs also responded to IL‐27 stimulation with divergent outcomes that were dependent on the inflammatory milieu of target cells. Conclusions These findings support the premise of autocrine/paracrine mechanisms involving IL‐27 in ADs under conditions of inflammatory stress that may link obesity with inflammatory diseases.