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Nanoformulated copper/zinc superoxide dismutase reduces adipose inflammation in obesity
Author(s) -
PerriotteOlson Curtis,
Adi Nikhil,
Manickam Devika S.,
Westwood Rachel A.,
Desouza Cyrus V.,
Natarajan Gopalakrishnan,
Crook Alexandra,
Kabanov Alexander V.,
Saraswathi Viswanathan
Publication year - 2016
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.21348
Subject(s) - inflammation , oxidative stress , endocrinology , adipose tissue , medicine , superoxide dismutase , proinflammatory cytokine , white adipose tissue , chemistry
Objective An intimate association exists between oxidative stress and inflammation. Because adipose tissue (AT) inflammation is intricately linked to metabolic disorders, it was hypothesized that reducing oxidative stress would be effective in ameliorating AT inflammation in obesity. Methods Wild‐type mice were fed a high‐fat diet (HF) for 8 weeks followed by a 2‐week treatment with nanoformulated copper/zinc superoxide dismutase (NanoSOD). The mice were divided into: 1) chow diet, 2) HF, and 3) HF + NanoSOD. Results The HF + NanoSOD‐treated mice showed a significant decrease in plasma and liver triglycerides when compared with HF‐fed mice. Interestingly, NanoSOD reduced the expression of macrophage and inflammatory markers in visceral AT (VAT) and stromal cells derived from VAT. Moreover, the activation of proinflammatory signaling pathways, in particular, the extracellular signal‐regulated kinases, was blunted in VAT on NanoSOD treatment. However, markers of oxidative stress were not altered significantly in the HF + NanoSOD group in the experimental conditions. Pretreatment of either macrophages or adipocytes significantly reduced the inflammatory response invoked in an in vitro coculture system, further supporting the role of NanoSOD in inhibiting obesity‐linked inflammation. Conclusions This data suggest that NanoSOD is effective not only in reducing AT macrophage accumulation and AT inflammation but also in promoting triglyceride metabolism in obesity.