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Jejunum–ileum circuit procedure improves glucose metabolism in diabetic rats independent of weight loss
Author(s) -
Wang Yanmin,
Zhang Xiang,
Liu Teng,
Zhong Mingwei,
Wan Houmin,
Liu Shaozhuang,
Zhang Guangyong,
Kassab Ghassan S.,
Hu Sanyuan
Publication year - 2016
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.21339
Subject(s) - glut2 , medicine , endocrinology , glucagon , leptin , peptide yy , diabetes mellitus , ileum , insulin , hormone , phosphoenolpyruvate carboxykinase , glucose transporter , biology , neuropeptide y receptor , neuropeptide , obesity , biochemistry , enzyme , receptor
Objective To introduce a lower‐risk novel surgical procedure to achieve diabetes reversal along with associated hormonal changes. Methods Diabetic rats were randomly assigned to jejunum–ileum circuit (JIC), sham‐JIC, ileal interposition (IT), and sham‐IT groups. The JIC group included two subgroups: short (JIC‐S) and long (JIC‐L), based on the length between anastomosis and Treitz ligament ( L AT ). The body weight, food intake, blood glucose, glucose and insulin tolerance, and gut hormones were measured. The liver gene expression of glucose transporter 2 (GLUT2) and protein expression of glucose‐6‐phosphatase (G6P) and phosphoenolpyruvate carboxykinase (PKC) were also measured. Following a dye infusion, nutrient delivery was measured at termination day. Results Compared to sham‐JIC group, JIC‐S group did not reduce body weight or food intake but significantly improved glucose tolerance and insulin resistance. With fast chyme transit, JIC‐S not only promoted the secretion of insulin, glucagon‐like peptide 1, and peptide YY and decreased leptin, but also upregulated hepatic GLUT2 and downregulated hepatic G6P and PKC. JIC‐L group, however, failed to achieve remission of diabetes. Conclusion JIC‐S relieves diabetes independent of weight loss, as it promotes the secretion of anti‐diabetic hormones and inhibits hepatic glucose production. The prolonging of L AT , however, diminishes the hypoglycemic effect.