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Altered intestinal neuroendocrine gene expression in humans with obesity
Author(s) -
Ritze Yvonne,
Hengelhaupt Christina,
Bárdos Gyöngyi,
Ernst Barbara,
Thurnheer Martin,
D'Haese Jan G.,
Bischoff Stephan C.,
Schultes Bernd
Publication year - 2015
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.21253
Subject(s) - ghrelin , endocrinology , medicine , leptin , jejunum , peptide yy , serotonergic , neuropeptide y receptor , cholecystokinin , hormone , tryptophan hydroxylase , serotonin transporter , biology , obesity , serotonin , receptor , neuropeptide
Objective Gastrointestinal hormones are critically involved in the regulation of food intake and body weight. Previous studies support an interplay between gastrointestinal hormones and the serotonergic system. This study explored intestinal neuroendocrine expression patterns in humans with obesity versus nonobese humans. Methods Jejunum samples were collected from 164 humans with obesity (120 women; BMI (mean ± SD): 43.5 ± 6.6 kg/m 2 ) while they underwent Roux‐en‐Y gastric bypass surgery and from 18 nonobese humans (7 women; BMI: 23.5 ± 3.0 kg/m 2 ) undergoing distinct intestinal surgeries. mRNA expression of cholecystokinin ( CCK ), peptide YY3‐36 ( PYY ), nesfatin1 , ghrelin , ghrelin O‐acyltransferase ( GOAT ), leptin , leptin receptor ( leptinR ), glucagon‐like‐peptide 1 receptor ( GLP1R ), serotonin transporter ( SERT ), tryptophan hydroxylase 1 ( TPH1 ), and serotonin receptor 3A ( 5HT 3A R ) was determined with qRT‐PCR. Ghrelin and GOAT protein expression was quantified using immunohistological stainings. Statistical analyses were performed with SPSS. Results Jejunum samples from humans with obesity showed a higher expression of GOAT (mRNA and protein), TPH1 , and SERT mRNA compared with the nonobese humans (all P < 0.05). Positive correlations were observed between TPH1 , CCK , PYY, and nesfatin1 in nonobese and GOAT, ghrelin, TPH1 , SERT , CCK , and PYY in humans with obesity (all P < 0.01). Conclusions Our top‐down approach substantiates the dysregulation of jejunal neuroendocrine hormones in obesity.