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Late effects of sleep restriction: Potentiating weight gain and insulin resistance arising from a high‐fat diet in mice
Author(s) -
de Oliveira Edson Mendes,
Visniauskas Bruna,
Sandri Silvana,
Migliorini Silene,
Andersen Monica Levy,
Tufik Sergio,
Fock Ricardo Ambrósio,
Chagas Jair Ribeiro,
Campa Ana
Publication year - 2015
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.20970
Subject(s) - endocrinology , medicine , insulin resistance , resistin , adipose tissue , weight gain , insulin , obesity , type 2 diabetes , biology , diabetes mellitus , body weight , adiponectin
Objective Epidemiological studies show the association of sleep restriction (SR) with obesity and insulin resistance. Experimental studies are limited to the concurrent or short‐term effects of SR. Here, we examined the late effects of SR regarding weight gain and metabolic alterations induced by a high‐fat diet (HFD). Methods C57BL/6 mice were subjected to a multiple platform method of SR for 15 days, 21 h daily, followed by 6 weeks of a 30% HFD. Results Just after SR, serum insulin and resistin concentrations were increased and glycerol content decreased. In addition, resistin, TNF‐α, and IL‐6 mRNA expression were notably increased in epididymal fat. At the end of the HFD period, mice previously submitted to SR gained more weight (32.3±1.0 vs. 29.4±0.7 g) with increased subcutaneous fat mass, had increments in the expression of the adipogenic genes PPARγ , C/EBPα , and C/EBPβ , and had macrophage infiltration in the epididymal adipose tissue. Furthermore, enhanced glucose tolerance and insulin resistance were also observed. Conclusions The consequences of SR may last for a long period, characterizing SR as a predisposing factor for weight gain and insulin resistance. Metabolic changes during SR seem to prime adipose tissue, aggravating the harmful effects of diet‐induced obesity.