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Intrahepatic fat content correlates with soluble CD 163 in relation to weight loss induced by R oux‐en‐ Y gastric bypass
Author(s) -
Fjeldborg Karen,
Pedersen Steen B.,
Møller Holger J.,
Rask Peter,
Danielsen Allan Vestergaard,
StødkildeJørgensen Hans,
Richelsen Bjørn
Publication year - 2015
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.20942
Subject(s) - cd163 , adipose tissue , weight loss , gastric bypass , medicine , fat distribution , gastroenterology , chemistry , obesity , endocrinology , macrophage , biochemistry , in vitro
Objective Soluble CD163 (sCD163) is a new marker of obesity‐related metabolic complications. sCD163 and CD163 mRNA were investigated in relation to the fat distribution at baseline and 12 months after Roux‐en‐Y gastric bypass (RYGB). Methods Thirty‐one obese subjects (BMI: 42.3 ± 4.7 kg/m 2 ) were enrolled. Subcutaneous (SAT) and visceral adipose tissue (VAT) volume were determined by MRI, intrahepatic lipid content (IHL) by MR‐spectroscopy, and body composition by DXA. Fasting blood samples and adipose tissue samples were obtained, and ELISA and RT‐PCR were performed. Results RYGB‐induced weight loss (36 ± 11 kg) was accompanied by a significant reduction in sCD163 (2.1 ± 0.8 mg/l vs. 1.7 ± 0.7 mg/l), SAT, VAT, and IHL (all, P < 0.001). At baseline, sCD163 was associated with VAT ( r = 0.40, P < 0.05) but not with SAT or IHL. Moreover, CD163 mRNA was significantly upregulated in VAT compared with SAT at baseline ( P < 0.05) and significantly downregulated in SAT after RYGB ( P < 0.001). ΔsCD163 was significantly associated with ΔIHL after RYGB compared with baseline ( r = 0.40, P < 0.05). Conclusions RYGB‐induced weight loss results in a reduction of sCD163 and CD163 mRNA. The association between ΔsCD163 and ΔIHL may reflect a reduction in sCD163‐producing Kupffer cells in the liver. Moreover, sCD163 may be a marker of “unhealthy” fat distribution in obese subjects.