Premium
Effect of d ‐fagomine on excreted enterobacteria and weight gain in rats fed a high‐fat high‐sucrose diet
Author(s) -
RamosRomero Sara,
MolinarToribio Eunice,
Gómez Livia,
PérezJiménez Jara,
Casado Marta,
Clapés Pere,
Piña Benjamín,
Torres Josep Lluís
Publication year - 2014
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.20640
Subject(s) - escherichia coli , feces , in vivo , bacteria , biology , endocrinology , medicine , microbiology and biotechnology , biochemistry , gene , genetics
Objective Becoming overweight has been related to elevated levels of Enterobacteriales in the gut. d‐ Fagomine is an iminosugar that has been shown to selectively agglutinate Enterobacteriales in vitro . The goal of this work is to establish whether d ‐fagomine exerts a similar effect in vivo and whether this has any downstream consequences on weight gain. Methods The rats were fed a high‐fat high‐sucrose diet (HFHS) supplemented with d ‐fagomine (or not; for comparison) or a standard diet for 5 weeks. The levels of total bacteria, Enterobacteriales and Escherichia coli were determined in fecal samples by performing quantitative real‐time polymerase chain reactions on DNA. Results Whereas the total levels of bacteria were independent of the diet, rats fed HFHS (without d ‐fagomine) excreted significantly higher proportions of Enterobacteriales and E. coli than those fed a standard diet. The levels of Enterobacteriales and E. coli of the rats given HFHS with d ‐fagomine were similar to those of the rats fed a standard diet. Compared to the standard group, rats fed HFHS with d ‐fagomine gained significantly less weight (15.3%) than those fed HFHS (20.9%). Conclusion d ‐Fagomine reduces the amount of Enterobacteriales excreted by rats fed HFHS and this may help to avert becoming obese.