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Increased β‐oxidation with improved glucose uptake capacity in adipose tissue from obese after weight loss and maintenance
Author(s) -
Bouwman Freek G.,
Wang Ping,
van Baak Marleen,
Saris Wim H.M.,
Mariman Edwin C.M.
Publication year - 2014
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1002/oby.20359
Subject(s) - medicine , adipose tissue , endocrinology , weight loss , white adipose tissue , triglyceride , lipoprotein lipase , biology , chemistry , obesity , cholesterol
Objective We investigated protein markers for pathways of the fatty acids (FAs) and glucose metabolism in human adipose tissue after a weight loss program by calorie restriction. Methods Overweight/obese subjects underwent an intervention of 5 weeks of a very low‐calorie diet followed by a 3‐week weight maintenance diet. Abdominal subcutaneous adipose tissue biopsies were sampled before and after the intervention. Seventeen target proteins as markers of metabolic pathways for the uptake and handling of FAs and glucose were quantified by Western blotting and 11 were retrieved from previous proteomics work. Correlation coefficients were calculated among changes of these proteins. Results Short‐chain 3‐hydroxyacyl‐CoA dehydrogenase, catalase, fatty acid translocase, fatty acid transporter protein 3, adipose triglyceride lipase, fatty acid‐binding protein 4, aldolase‐C, tubulin‐β‐5, and annexin A2 changed significantly, and lipoprotein lipase, perilipin 1, and hormone‐sensitive lipase tended to change. On an average, increased glucose transporter type 4 translocation was observed, supported by a consistent increase of tyr‐24 phosphorylated annexin A2. Conclusions Our findings suggest that after weight loss by calorie restriction and a short period of maintenance, adipose tissue has an increased capacity for glucose uptake, and lipid mobilization and oxidation. Such metabolic profile may relate to the health benefit of weight loss.