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Effects of Sodium Hydroxide Treatment and Ultrafiltration on the Removal of Humic Contaminants from Archaeological Bone
Author(s) -
Szpak P.,
Krippner K.,
Richards M. P.
Publication year - 2017
Publication title -
international journal of osteoarchaeology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 60
eISSN - 1099-1212
pISSN - 1047-482X
DOI - 10.1002/oa.2630
Subject(s) - ultrafiltration (renal) , sodium hydroxide , contamination , chemistry , hydroxide , sodium , environmental chemistry , extraction (chemistry) , nuclear chemistry , chromatography , inorganic chemistry , biology , organic chemistry , ecology
This study compared bone collagen extraction techniques that included treatment with sodium hydroxide and 30 kDa ultrafilters using a set of well‐preserved, humic‐contaminated archaeological marine mammal bones. Treatment with sodium hydroxide was effective at removing humic contaminants from archaeological bone, although yields were significantly decreased. Yields were also significantly decreased by ultrafiltration although this study produced no evidence that 30 kDa ultrafilters were effective at selectively removing humic contaminants from archaeological bone. The combination of sodium hydroxide treatment and ultrafiltration did not produce superior results to the treatment involving only sodium hydroxide. Archaeological samples exhibiting darker colouration indicative of humic contamination should be treated with sodium hydroxide to remove these contaminants. Copyright © 2017 John Wiley & Sons, Ltd.