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Immunological diagnosis of multiple myeloma in a medieval bone
Author(s) -
Cattaneo C.,
Gelsthorpe K.,
Phillips P.,
Waldron T.,
Booth J. R.,
Sokol R. J.
Publication year - 1994
Publication title -
international journal of osteoarchaeology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 60
eISSN - 1099-1212
pISSN - 1047-482X
DOI - 10.1002/oa.1390040102
Subject(s) - multiple myeloma , hyperviscosity syndrome , hyperviscosity , antibody , medicine , monoclonal antibody , immunoglobulin a , pathology , immunology , microbiology and biotechnology , chemistry , immunoglobulin g , biology , blood viscosity
An inhibition enzyme‐linked immunosorbent assay using monoclonal antibodies has been used to confirm the diagnosis of IgA type multiple myeloma in a small fragment (< 10 g) of cranium from a medieval female. Further testing by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis and immunoblotting showed that the IgA was mainly polymeric, suggesting that the individual would have suffered from a hyperviscosity syndrome. This is the first time that immunological assays have been used to diagnose multiple myeloma in ancient human tissue and have permitted speculation on associated clinical symptoms.