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Dimethyl sulfone in human cerebrospinal fluid and blood plasma confirmed by one‐dimensional 1 H and two‐dimensional 1 H‐ 13 C NMR
Author(s) -
Engelke Udo F. H.,
Tangerman Albert,
Willemsen Michèl A. A. P.,
Moskau Detlef,
Loss Sandra,
Mudd S. Harvey,
Wevers Ron A.
Publication year - 2005
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.966
Subject(s) - chemistry , cerebrospinal fluid , methionine adenosyltransferase , metabolite , methanethiol , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , nmr spectra database , blood plasma , sulfone , resonance (particle physics) , nuclear magnetic resonance , chromatography , methionine , spectral line , stereochemistry , biochemistry , organic chemistry , medicine , sulfur , physics , astronomy , particle physics , amino acid
1 H‐NMR spectroscopy at 500 MHz was used to confirm that a previously unidentified singlet resonance at 3.14 ppm in the spectra of cerebrospinal fluid and plasma samples corresponds to dimethyl sulfone (DMSO 2 ). A triple resonance inverse cryogenic NMR probe, with pre‐amplifier and the RF‐coils cooled to low temperature, was used to obtain an 1 H‐ 13 C HSQC spectrum of CSF containing 8 µ M (753 ng/ml) DMSO 2 . The 1 H‐ 13 C correlation signal for DMSO 2 was assigned by comparison with the spectrum from an authentic reference sample. In plasma and CSF from healthy controls, the concentration of DMSO 2 ranged between 0 and 25 µmol/l. The concentration of DMSO 2 in plasma from three of four patients with severe methionine adenosyltransferase I/III (MAT I/III) deficiency was about twice the maximum observed for controls. Thus, DMSO 2 occurs as a regular metabolite at low micromolar concentrations in cerebrospinal fluid and plasma. It derives from dietary sources, from intestinal bacterial metabolism and from human endogenous methanethiol metabolism. Copyright © 2005 John Wiley & Sons, Ltd.