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Functional mapping of neural pathways in rodent brain in vivo using manganese‐enhanced three‐dimensional magnetic resonance imaging
Author(s) -
Watanabe Takashi,
Frahm Jens,
Michaelis Thomas
Publication year - 2004
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.937
Subject(s) - neuroscience , hippocampal formation , olfactory bulb , human brain , in vivo , cerebellum , central nervous system , inferior colliculus , magnetic resonance imaging , functional magnetic resonance imaging , biology , medicine , microbiology and biotechnology , nucleus , radiology
This work presents three‐dimensional MRI studies of rodent brain in vivo after focal and systemic administration of MnCl 2 . Particular emphasis is paid to the morphology and dynamics of Mn 2+ ‐induced MRI signal enhancements, and the physiological mechanisms underlying cerebral Mn 2+ uptake and distribution. It turns out that intravitreal and intrahippocampal injections of MnCl 2 emerge as useful tools for a delineation of major axonal connections in the intact central nervous system. Subcutaneous administrations may be exploited to highlight regions involved in fundamental brain functions such as the olfactory bulb, inferior colliculus, cerebellum and hippocampal formation. Specific insights into the processes supporting cerebral Mn 2+ accumulation may be obtained by intraventricular MnCl 2 injection as well as by pharmacologic modulation of, for example, hippocampal function. Taken together, Mn 2+ ‐enhanced MRI opens new ways for mapping functioning pathways in animal brain in vivo with applications ranging from assessments of transgenic animals to follow‐up studies of animal models of human brain disorders. Copyright © 2004 John Wiley & Sons, Ltd.