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Measurements of intracellular volumes by 59 Co and 2 H/ 1 H NMR and their physiological applications
Author(s) -
Askenasy Nadir,
Navon Gil
Publication year - 2005
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.908
Subject(s) - intracellular , nuclear magnetic resonance spectroscopy , context (archaeology) , intracellular ph , biophysics , chemistry , permeability (electromagnetism) , energy metabolism , membrane , metabolism , biochemistry , biology , paleontology , endocrinology , organic chemistry
Determination of the intracellular water volumes using NMR spectroscopy was performed using the NMR‐visible nuclei: 59 Co and 2 H or 1 H. Accurate measurement of intracellular water in cell suspensions and perfused organs is an important physiological parameter in the context of electrolyte homeostasis and energy metabolism, in particular when these parameters are monitored by non‐invasive NMR spectroscopy. Furthermore, repeated or continuous monitoring of intracellular water provided significant insights into the physiology of cardiac muscle and sarcolemmal membrane permeability and integrity. Copyright © 2005 John Wiley & Sons, Ltd.