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Quantification of water diffusion and relaxation times of human U87 tumors in a mouse model
Author(s) -
Sun Yanping,
Mulkern Robert V.,
Schmidt Karl,
Doshi Sameer,
Albert Mitchell S.,
Schmidt Nils O.,
Ziu Mateo,
Black Peter,
Carrol Rona,
Kieran Mark W.
Publication year - 2004
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.894
Subject(s) - diffusion , u87 , chemistry , relaxation (psychology) , nuclear magnetic resonance , biology , cancer research , neuroscience , glioma , physics , thermodynamics
Assessing the potential of anti‐cancer agents can be greatly facilitated by applying MRI methods to investigations with animal models. Quantitative diffusion imaging, T 1 , and T 2 measurements may offer valuable information for understanding properties of the tumor and for evaluating new therapeutic approaches. The human U87 high‐grade glial tumor is widely used for cancer investigations in orthotopic murine models. The physiological features of this model at the cellular and sub‐cellular level have not, however, been well characterized by MRI. In this study, we measured the diffusion, T 1 and T 2 characteristics of water in the human U87 tumor at 8.5 T in an orthotopic murine model in vivo and analyzed their detailed changes in the transition from the tumor core through the tumor periphery, and out to surrounding tissue using custom developed radial profile analysis software. For the tumor bearing mice ( n = 10), the mean average apparent diffusion coefficient (ADC) of the tumor core was 1.03 ± 0.02 ( × 10 −3 mm 2 /s), while in the contralateral normal brain it was 0.73 ± 0.03 ( × 10 −3 mm 2 /s). The mean T 1 in tumor was 2.03 ± 0.08 s and in normal brain tissue was 1.64 ± 0.06 s. The mean T 2 in tumor was 0.062 ± 0.002 s and in normal brain tissue was 0.048 ± 0.001 s. The mean ADC, T 1 and T 2 of the tumor compared to normal tissue were significantly different ( p < 0.005). Copyright © 2004 John Wiley & Sons, Ltd.