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1 H and 13 C HR‐MAS spectroscopy of intact biopsy samples ex vivo and in vivo 1 H MRS study of human high grade gliomas
Author(s) -
MartínezBisbal M. Carmen,
MartíBonmatí Luis,
Piquer José,
Revert Antonio,
Ferrer Pilar,
Llácer José L.,
Piotto Martial,
Assemat Olivier,
Celda Bernardo
Publication year - 2004
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.888
Subject(s) - in vivo , nuclear magnetic resonance , magic angle spinning , ex vivo , nuclear magnetic resonance spectroscopy , chemistry , in vivo magnetic resonance spectroscopy , biopsy , spectroscopy , analytical chemistry (journal) , magnetic resonance imaging , pathology , chromatography , biology , medicine , physics , microbiology and biotechnology , quantum mechanics , radiology
Abstract High‐resolution magic angle spinning (HR‐MAS) one‐ and two‐dimensional 1 H and 13 C nuclear magnetic resonance (NMR) spectroscopy has been used to study intact glioblastoma (GBM) brain tumour tissue. The results were compared with in vitro chemical extract and in vivo spectra. The resolution of 1 H one‐dimensional, 1 H TOCSY and 13 C HSQC HR‐MAS spectra is comparable to that obtained on perchloric extracts. 13 C HSQC HR‐MAS spectra have been particularly useful for the identification of 37 different metabolites in intact biopsy tumours, excluding water and DSS components. To our knowledge, this is the most detailed assignment of biochemical compounds obtained in intact human tissue, in particular in brain tumour tissue. Tissue degradation during the recording of the NMR experiment was avoided by keeping the sample at a temperature of 4°C. Detailed metabolical compositions of 10 GBM (six primary, two secondary and two unclassified) were obtained. A good correlation between ex vivo and in vivo MRS has been found. Copyright © 2004 John Wiley & Sons, Ltd.