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Correlation between the occurrence of 1 H‐MRS lipid signal, necrosis and lipid droplets during C6 rat glioma development
Author(s) -
Zoula Sonja,
Hérigault Gwénaël,
Ziegler Anne,
Farion Régine,
Décorps Michel,
Rémy Chantal
Publication year - 2003
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.831
Subject(s) - lipid droplet , necrosis , lipid accumulation , glioma , in vivo , fat necrosis , chemistry , lipid metabolism , pathology , nuclear magnetic resonance , biology , biochemistry , medicine , cancer research , physics , microbiology and biotechnology
The aim of this study was to investigate the possible correlation between the 1 H MRS mobile lipid signal, necrosis and lipid droplets in C6 rat glioma. First, the occurrence of necrosis and lipid droplets was determined during tumor development, by a histological analysis performed on 34 rats. Neither necrosis nor lipid droplets were observed before 18 days post‐implantation. At later stages of development, both necrosis and lipid droplets were apparent, the lipid droplets being mainly located within the necrotic areas. Using a second group of eight rats, a temporal correlation was evidenced between mobile lipid signal detected by in vivo single‐voxel one‐ (136 ms echo time) and two‐dimensional J ‐resolved 1 H MR spectroscopy, and the presence of necrosis and lipid droplets on the histological sections obtained from the brains of the same rats. Finally, spatial distribution of the mobile lipid signal was analyzed by chemical‐shift imaging performed on a third group of eight animals, at the end of the tumor growth. The spectroscopic image corresponding to the resonance of mobile lipids had its maximum intensity in the center of the tumor where necrotic regions were observed on the histological sections. These necrotic areas contained large amounts of lipid droplets. All these results suggest that mobile lipids detected in vivo by 1 H MRS (136 ms echo time) in C6 rat brain glioma arise mainly from lipid droplets located in necrosis. Copyright © 2003 John Wiley & Sons, Ltd.