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Leakage of metabolites from tissue biopsies can result in large errors in quantitation by MRS
Author(s) -
Bourne Roger,
Dzendrowskyj Theresa,
Mountford Carolyn
Publication year - 2003
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.815
Subject(s) - tissue sample , reproducibility , brain tissue , prostate , buffer (optical fiber) , pathology , biopsy , chemistry , chromatography , nuclear medicine , biomedical engineering , medicine , telecommunications , computer science , cancer
The leakage of metabolites from frozen and thawed tissue biopsies was measured semi‐quantitatively by high‐field (8.5 T) proton MRS. Human prostate and rat brain tissue specimens, frozen within 1 min of collection, lost significant and variable amounts of diagnostic metabolites immediately upon thawing. For prostate tissue 30–50% of initial total choline compounds, total creatines and citrate were detected in the collection buffer immediately after thawing. The widely used protocol for MR assessment of tissue biopsies, which involves washing of thawed tissue samples in fresh buffer, results in loss of large and unpredictable amounts of possibly diagnostic metabolites prior to MRS. This reduces the reproducibility of MR analysis of tissue biopsies and compromises the reliable identification of MR spectral patterns diagnostic of tissue pathology. The problem can be avoided by minimizing the volume of storage buffer, omitting tissue washing and performing MRS measurements on the tissue immersed in the original storage buffer. Copyright © 2003 John Wiley & Sons, Ltd.