Premium
High‐resolution magic angle spinning MRS of breast cancer tissue
Author(s) -
Sitter Beathe,
Sonnewald Ursula,
Spraul Manfred,
Fjösne Hans E.,
Gribbestad Ingrid S.
Publication year - 2002
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.775
Subject(s) - perchloric acid , breast cancer , breast tissue , nuclear magnetic resonance , high resolution , spectral line , resolution (logic) , human breast , chemistry , cancer , magic angle spinning , analytical chemistry (journal) , chromatography , medicine , nuclear magnetic resonance spectroscopy , physics , organic chemistry , computer science , remote sensing , astronomy , artificial intelligence , geology
High‐resolution magic angle spinning (HR MAS) may develop into a new diagnostic tool for studying intact tissue samples, and several types of cancer have been investigated with promising results. In this study HR MAS spectra of breast cancer tissue from 10 patients have been compared to conventional high‐resolution spectra of perchloric acid extracts of the same tissue type. The HR MAS spectra show resolution comparable to spectra of extracts, and two‐dimensional techniques lead to identification of a majority of the constituents. More than 30 different metabolites have been detected and assigned. To our knowledge this is the most detailed assignment of biochemical components in intact human breast tissue. The spectra of intact breast cancer tissue differ from perchloric acid extracts by the presence of lipids and fewer signals in the low field region. HR MAS analysis of intact breast tissue specimens is a rapid method, providing spectra with resolution where relative quantification of the majority of the detected metabolites is possible. Copyright © 2002 John Wiley & Sons, Ltd.