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Solution NMR in human embryo culture media as an option for assessment of embryo implantation potential
Author(s) -
Salmerón Ana M.,
Abreu Ana Cristina,
VilchesFerrón Miguel,
Fernández Ignacio
Publication year - 2021
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.4536
Subject(s) - embryo , embryo culture , chemistry , biology , microbiology and biotechnology , embryogenesis
NMR offers the potential to holistically screen hundreds of metabolites and has already proved to be a powerful technique able to provide a global picture of metabolic changes in a wide range of biological systems underlying complex and multifactorial matrixes. This review covers the literature until May 2020 centered on the early prediction of the viability of in vitro developed embryos using several analytical techniques, including NMR. Nowadays, the predominant non‐invasive technique for selecting viable embryos is based on morphology, where variables associated with the rate of cleavage and blastocyst formation are evaluated by the embryologist following standardized criteria that are somewhat subjective. This morphological approach is therefore inadequate for the prediction of embryo quality, and several studies have focused on developing new non‐invasive methods using molecular approaches based particularly on metabolomics. This review outlines the potential of NMR as one of these non‐invasive in vitro methods based on the analysis of spent embryo culture media.