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Molecular correlates of MRS‐based 31 phosphocreatine muscle resynthesis rate in healthy adults
Author(s) -
Darpolor Moses M.,
Singh Maninder,
Covington Jeffrey,
Hanet Sebastian,
Ravussin Eric,
Carmichael Owen T.
Publication year - 2021
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.4402
Subject(s) - phosphocreatine , skeletal muscle , nicotinamide phosphoribosyltransferase , medicine , biology , vastus lateralis muscle , endocrinology , nad+ kinase , energy metabolism , biochemistry , enzyme
Dynamic phosphorus MRS ( 31 P‐MRS) is a method used for in vivo studies of skeletal muscle energetics including measurements of phosphocreatine (PCr) resynthesis rate during recovery of submaximal exercise. However, the molecular events associated with the PCr resynthesis rate are still under debate. We assessed vastus lateralis PCr resynthesis rate from 31 P‐MRS spectra collected from healthy adults as part of the CALERIE II study (caloric restriction), and assessed associations between PCr resynthesis and muscle mitochondrial signature transcripts and proteins (NAMPT, NQO1, PGC‐1α, and SIRT1). Regression analysis indicated that higher concentration of nicotinamide phosphoribosyltransferase (NAMPT) protein, a mitochondrial capacity marker, was associated with faster PCr resynthesis. However, PCr resynthesis was not associated with greater physical fitness (VO 2 peak) or messenger ribonucleic acid levels of mitochondrial function markers such as NQO1, PGC‐1α, and SIRT1, suggesting that the impact of these molecular signatures on PCr resynthesis may be minimal in the context of an acute exercise bout. Together, these findings suggest that 31 P‐MRS based PCr resynthesis may represent a valid non‐invasive surrogate marker of mitochondrial NAMPT in human skeletal muscle.