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The hemodynamic and metabolic effects of spironolactone treatment in acute kidney injury assessed by hyperpolarized MRI
Author(s) -
Lindhardt Jakob Lykke,
Nielsen Per Mose,
Hansen Esben Søvsø Szocska,
Qi Haiyun,
Tougaard Rasmus Stilling,
Mariager Christian Østergaard,
Bertelsen Lotte Bonde,
Kim Won Yong,
Laustsen Christoffer
Publication year - 2020
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.4371
Subject(s) - spironolactone , kidney , medicine , endocrinology , ischemia , urea , perfusion , renal blood flow , urology , chemistry , aldosterone , biochemistry
Renal ischemia‐reperfusion injury (IRI) is one of the most common types of acute kidney injury. Spironolactone has shown promising kidney protective effects in renal IRI in rats. We investigated the hemodynamic and metabolic effects of spironolactone (100 mg/kg) administered immediately after 40 min unilateral kidney ischemia in rats. Hyperpolarized MRI using co‐polarized [1‐ 13 C]pyruvate and [ 13 C, 15 N 2 ]urea as well as 1 H dynamic contrast‐enhanced (DCE) MRI was performed 24 h after induction of ischemia. We found a significant decrease in renal blood flow (RBF) in the ischemic kidney compared with the contralateral one measured using DCE and [ 13 C, 15 N 2 ]urea. The RBF measured using [1‐ 13 C]pyruvate and [ 13 C, 15 N 2 ]urea was significantly altered by spironolactone. The RBFs in the ischemic kidney compared with the contralateral kidney were decreased similarly as measured using both [ 13 C, 15 N 2 ]urea and [1‐ 13 C]pyruvate in the spironolactone‐treated group. Spironolactone treatment increased the perfusion‐corrected pyruvate metabolism by 54% in both the ischemic and contralateral kidney. Furthermore, we showed a correlation between vascular permeability using a histological Evans blue analysis and the ratio of the volumes of distribution (VoDs), ie VoD‐[ 13 C, 15 N 2 ]urea/VoD‐[1‐ 13 C]pyruvate. This suggests that [ 13 C, 15 N 2 ]urea/[1‐ 13 C]pyruvate VoD ratio may be a novel indicator of renal vascular permeability associated with renal damage in rodents.